The performance and application of TMB:
3,3',5,5'-Tetramethylbenzidine(TMB) is a new-type safe color primary reagent.Compared with some traditional color primary reagents such as benzidine, o-tolidine and o-diaminobenzene,the merits of TMB are high sensitive detection and excellent stability. And as a result of zoopery and Ames which is to experiment self-acting variability of bacteria,showing that TMB is a kind of non-cancerogen and non-mutagen ,and can be used safely. Currently,TMB is taking the place of benzidine which is a strong cancerogen and other ramifications of benzidine which are carcinogenic step by step.TMB can be applied in areas like clinical assay, forensic analysis, criminal investigation and environmental detection,etc.As a new zymolyte of peroxidase,TMB has wide applications in enzyme immunoassay (EIA) and enzyme-linked-immunosorbent serologic assay (ELISA).
The main items of TMB’s applications are:
1、making up test paper of uranalysis;
2、detection of hepatitis virus;
3、detection of gravidity;
4、rapid determination of glucose,hemoglobin and albumin in blood and urine;
5、occult blood test of excrement;
6、to detect the value of granulocyte in blood;
7、detection of steroid and sex hormone;
8、determination of enzyme activity;
9、analysis and detection of antigen, antibody and germ plasm;
10、rapid determination of alcoholicity in saliva;
11、pigmentation of biological specimen;
12、quality test of water and detection of fingerprint rendering;
13、detection of AIDS virus.
14、Horseradish Peroxidase Color Development Solution for ELISA.