A water soluble pH dependent bifunctional fluorogenic reagent for the sensitive and specific detection of protein thiols. ABD-F is highly reactive and specific for protein thiols, and its selectivity is superior to that of other sulfhydryl-reactive fluorophores such as fluorescein maleimide, 5-iodoacetamidofluorescein, 5-I-AEDANS, dansylaziridine, and bimane.
Fluorescence: max Abs. l = 385nm, max Em. l = 520nm; Molar Abs. > 4.5x103 (ca. 315nm)
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